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Heinz Roland Jakobi

University of São Paulo, Brazil

Title: Validation of human brucellosis test diagnostic: Test Rose Bengal [TRB], ELISA (IGM and IGG) and PCR Real Time Multiplex


Biography: Heinz Roland Jakobi


Brucellosis is a universal and re-emerging anthropozoonosis caused by bacteria of the genus Brucella as a diagnostic method of human brucellosis blood culture is the gold standard of diagnosis but difficult practical applicability. The Ministry of Health recommends using the Rose Bengal test [RBT] and ELISA [IGG and IGM]. The polymerase chain reaction [PCR] is an efficient and accurate method of DNA amplification. These laboratory tests have few studies of specificity and sensitivity and accuracy and do not yet have national validation. Objective is to validate diagnostic tests of human brucellosis, research the accuracy, specificity and sensitivity of serological tests Rose Bengal, ELISA and Real Time PCR Multiplex PCR. It is a prospective cohort study analyzing the tests conducted in Rondonia Central Laboratory [LACEN-RO] and rebuttal at the Institute Adolf Lutz [IAL-SP]. The brucellosis tests will be applied to a control group of non-endemic area, São Paulo. Mishandled samples will be deleted. The true-positive tests, true-negative, false-positive and false-negative will be determined by evaluating the accuracy of the tests and applied to calculate Likelihood Ratio [RV] and the ROC curve and the Bayes theorem establishing prevalence, sensitivity, specificity and predictive value. The data collected will be subjected to analysis in IBM SPSS Statistics Standard version 20® spreadsheet.