Biotechnology World Convention
Sao Paulo, Brazil
Heinz Roland Jakobi
University of São Paulo, Brazil
Title: Serological and molecular tests for the diagnosis of human brucellosis in workers in slaughterhouses in Rondonia in the Brazilian Amazon
Biography: Heinz Roland Jakobi
Brucellosis is a universal and re-emerging anthropozoonosis caused by bacteria of the genus Brucella spp., of the ten recognized species of Brucella spp., four are pathogenic to humans; melitensis, suis, abortus and canis. According to some authors to B. melitensis is not found in Brazil. As a diagnostic method of human brucellosis blood culture is the gold standard of diagnosis but difficult practical applicability. The Ministry of Health recommends using the Rose Bengal test [RBT] and ELISA [IGG and IGM]. The polymerase chain reaction [PCR] is an efficient and accurate method of DNA amplification. The Amazon region has not owned epidemiological data and Rondonia is endemic illness and compulsory health notification being recorded cases due to the presence of infected animals, especially in refrigerators workers. Objective is to develop and validate serologic and molecular tests for the diagnosis of human brucellosis in refrigerators workers in Rondonia. It is an observational prospective cohort study of human brucellosis in Rondonia performing laboratory tests Rose Bengal, ELISA and PCR in Rondonia State Central Laboratory [LACEN-RO] with rebuttal in Adolf Lutz Institute [ITA-SP]. Samples mishandled sera will be deleted. The data collected will be subjected to analysis in Microsoft Office Excel® spreadsheet and IBM SPSS Statistics Standard version 20®.